Samuel Bunga

samuelbunga.github.io

bunga.s@northeastern.edu

samuelbunga

samuelbunga

thebluenerve

San Diego, Massachusetts 92122 USA

857-701-9190

Interests

Single Cell Genomics , Network inference , Bioinformatics software development

Skills

Programming/Development: Python, JavaScript, R, Shell Scripting, Nextflow, PHP, CI/CD, Git, AWS

Education

Northeastern University, Boston, MA

M.S., Bioinformatics

  • Computational Methods – 1 and 2, Bioinformatics Programming, Machine Learning, Data Analytics

August 2018 - May 2020

Work

Bioinformatics Scientist 2,

Illumina, Inc., San Diego, CA

  • Developed bioinformatics infectious disease applications for the MiSeq i100 instrument
  • Possess extensive experience in designing, developing, and optimizing Nextflow pipelines for high-throughput analysis workflows
  • Contributed to the development of multiple bioinformatics applications, including GISAID Uploader, Microbiome Metagenomics, Microbial Enrichment Analysis, DRAGEN COVID Lineage, DRAGEN Viral Lineage, and DRAGEN Targeted Microbial
  • Designed and implemented reusable Python modules integrated across various DRAGEN pipelines, enhancing functionality and performance of internal tools

February 2022 - Present

Bioinformatics Software Developer, Harvard Program in Therapeutic Science (HiTS)

Harvard Medical School, Boston, MA

  • Developed Neuro Immune interactome pipeline using Nextflow and Python to study the interactions in different mouse pain models between Immune cell ligands and Neuron receptors using INDRA network and cellphonedb (github.com/indralab/panacea_indra)
  • Designed and developed a workflows using COSMOS to integrate and connect data from different omic (Phospho-proteomics and Transcriptomics) datasets to make network level causal reasoning in CFA and Naïve sciatic nerve samples
  • Built preprocessing workflows for inDrops and 10x single-cell RNA-Seq data using bcbio and CellRanger, respectively
  • Maintained and improved bulk and single-cell RNA-Seq pipelines for the Boston Children’s Hospital bioinformatics core
  • Contributed to and enhanced CXP-lite, a neuronal activity profiling tool, significantly improving performance and reducing manual effort for the Woolf Lab

August 2020 - February 2022

Graduate Teaching Assistant, Computational Methods-2

Northeastern University, Boston, MA

  • Led weekly lab sessions and office hours, supporting a cohort of 30 students in computational techniques and programming concepts
  • Provided individualized academic mentorship and guidance on course material and coding assignments
  • Conduct and evaluate student quizzes and assignments

September 2019 - April 2020

Research Intern, Bhasin Lab

Harvard Medical School, Boston, MA

  • Designed and developed Single Cell Wizard, a free web application for processing 10X, CEL-Seq, and Drop-Seq single-cell RNA-Seq data.
  • Developed home brew tools for Single Cell Wizard pipeline like FASTQ demultiplexer and FASTQ cleaner to remove singleton nucleotides and low-quality reads from each sample
  • Assembled and created a Database for Gene expression markers that define cell types which can be used to annotate clusters of single cells via Gene Set Enrichment Analysis

May 2019 - December 2019

Project Experience

Single Cell Wizard, Bhasin Lab

bhasinlab.us/scw_page/scwiz.php

Single Cell RNA-seq pre/post processing pipeline

  • Single Cell Wizard is a comprehensive Single Cell RNA-Seq processing web-application which is a totally automated pipeline that can take raw FASTQ files, process them to generate feature barcode matrices and perform pre-processing, data normalization, quality control to differentially expressed genes, annotation of clusters and final visualization

August 2019 - December 2019

Indian Ayurvedic Bioflavonoids Database, Northeastern University

  • Compiled over 100 plant metabolites in Ayurvedic formulations and forming up an interactome which could serve as a lead for potential drug targets that can be taken up for clinical trials

January 2019 - March 2019

References

Available upon request